Adjusting The Field Diaphragm - Nikon Eclipse Ni-E Instruction Manual

Upright motorized microscope (focusing nosepiece system)
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10

Adjusting the Field Diaphragm

The field diaphragm is used to restrict illumination to the area of
the sample being viewed.
Operate the DIA field diaphragm button to change the size of the
field diaphragm.
Press the left side (
mark) of the DIA field diaphragm button to
open, and the right side (
For Ni-E, the field diaphragm can be adjusted from 1.5 mm to
30.6 mm.
For normal observations, the size of the field diaphragm should
almost circumscribe the field diaphragm.
Operation on DS-L3 (See "20.2 Microscope Control".)
Proper size of the field diaphragm
Usually, the size is optimal when it almost circumscribes the field of view. Opening the field diaphragm too much and
illuminating a broader area than necessary will result in stray light entering the field of view, generating flare and
reducing the image contrast. In addition, the sample will become decolorized over a wider area.
Field diaphragm's adjustment timing
Be sure to adjust the aperture diaphragm each time you change the objective.
Chapter 3
mark) to close the diaphragm.
Individual Operations
DIA field diaphragm
button
Adjusting the field diaphragm
P A _ _ 2 x _ _ _ _
B F
_ _ _ _
Right size of the field diaphragm
61
ND
ND
NCB
8
32
11
OUT
IN
Z :
_ _ _ _ _
0 . 0 0 0 u m
A S 3 0 . 6 m m
_ _
F S 3 0 . 0 m m

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