Table Of Contents - Nikon Eclipse Ni-E Instruction Manual

Contents
Introduction ......................................................................................................................... i
Summary of Contents ....................................................................................................... iii
Safety Precautions............................................................................................................ vi
Notes on Handling the Product......................................................................................... xi
Components............................................................................................. 1
1
Microscopy Operations........................................................................... 7
1
2
3
4
Individual Operations ............................................................................ 41
1
2
3
4
5
6
7
8
9
Contents
Contents of the Manual ....................................................................................................ii
Symbols Used in This Manual ..........................................................................................ii
WARNING and CAUTION Symbols ................................................................................vi
Meaning of Symbols Used on the Product ......................................................................vi
WARNING ................................................................................................................ vii
CAUTION ..................................................................................................................ix
Ni-E Focusing Nosepiece System Basic Configuration ............................................2
1.1 Components ....................................................................................................... 2
1.2 Controls .............................................................................................................. 3
1.3 Operation Buttons .............................................................................................. 4
1.4 Connectors ......................................................................................................... 5
Before Microscopy ....................................................................................................8
Operation Flowchart................................................................................................10
IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure ....12
Epi-fluorescence Microscopy Procedure.................................................................29
Display Panel Details ..............................................................................................42
Using Operation Buttons on Ni-E ............................................................................45
2.1 Front Operation Button..................................................................................... 45
2.2 Right Operation Button..................................................................................... 46
2.3 Left Operation Button ....................................................................................... 47
Adjusting the Brightness of a Diascopic Image.......................................................48
3.1 Adjustment by Lamp Voltage ........................................................................... 48
3.2 Adjustment with ND Filters............................................................................... 49
Focusing on the Sample (Vertical Objective Movement) ........................................50
4.1 Proper Focusing Procedure ............................................................................. 50
4.2 Focus Knobs on the Main Body ....................................................................... 51
4.3 Resetting the Z-axis Coordinate....................................................................... 52
4.4 Refocusing ....................................................................................................... 53
Bringing the Target into the Optical Path (Horizontal Stage Movement) .................54
5.1 Standard Stage Operation................................................................................ 54
5.2 Operating the Motorized XY Stage................................................................... 55
Adjusting the Diopter...............................................................................................56
Focusing and Centering the Condenser .................................................................57
Adjusting the Aperture Diaphragm ..........................................................................58
Using the Condenser ..............................................................................................59
iv