Nikon Eclipse Ni-E Instruction Manual
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Nikon Eclipse Ni-E Instruction Manual

Nikon Eclipse Ni-E Instruction Manual

Upright motorized microscope
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M571 E
11.8.NF.1 (2/2)
Upright Motorized Microscope
Instruction Manual
Assembly/Maintenance

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  • Page 1 M571 E 11.8.NF.1 (2/2) Upright Motorized Microscope Instruction Manual Assembly/Maintenance...

  • Page 3: Introduction

    Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of the Nikon ECLIPSE Ni-E microscope. To ensure correct usage, read this manual carefully before operating this product. ● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

  • Page 4: Contents Of The Manual

    Introduction Contents of the Manual The instruction manual for ECLIPSE Ni-E is provided in two volumes.  Operation  Assembly/Maintenance (This manual) Safety Precautions Assembly Components Troubleshooting Microscopy Operations Maintenance and Storage Before Microscopy Specifications Operation Flowchart Before reading the “Assembly/Maintenance” manual, Bright/Dark-field Microscopy Procedure read the “Safety Precautions”...

  • Page 5: Summary Of Contents

    Summary of Contents Summary of Contents (See the next page for the detailed contents.) Assembly Introduction Contents of the Manual Symbols Used in This Manual Troubleshooting Maintenance and Storage Specifications...

  • Page 6: Table Of Contents

    Introduction ......................... i Contents of the Manual ....................ii Symbols Used in This Manual ..................ii Summary of Contents ....................... iii Assembly....................1 ECLIPSE Ni-E System Configuration................2 Components List .......................4 Assembly Method......................6 Introduction : Motorized Device Combination ..............6 Check input voltage.................... 7 Unfasten the elevating section.
  • Page 7 Contents [24] Replace the ND filter ..................34 [25] Connect the motorized unit cable..............34 [26] Connect the power cord................... 35 Troubleshooting..................37 Optical System and Operation ................38 General......................38 Epi-fluorescence Microscopy ................42 Differential Interference Contrast Microscopy..........43 Phase Contrast Microscopy ................
  • Page 8 Contents...

  • Page 9: Assembly

    Chapter Assembly This chapter contains the system configuration diagram and components list for Ni-E, and instructions on assembling the system. Read appropriate notes such as CAUTION “9 Cautions on assembling and installing the product” at the beginning of the separately provided instruction manual “Operation” or “4 Installation location” in “Notes on Handling the Product” in the same manual before you work on assembling.

  • Page 10: Eclipse Ni-E System Configuration

    Chapter 1 Assembly ECLIPSE Ni-E System Configuration ENG mount camera Camera cable C mount camera Various adapters to DS-U3 or photomicrography device DS-L3 DS camera head Centering CFI eyepiece telescope TV adapter DSC port for ergonomic Tube adapter for binocular tube...
  • Page 11 Chapter 1 Assembly Connected to connector box with a motorized shutter Ni-E main body cable To motorized Substage for rotatable XY stage ceramic coated controller stage/motorized XY stage using a driver Motorized Motorized shutter cable shutter adapter To control box A using the extension cable Control...

  • Page 12: Components List

    The ECLIPSE Ni-E components are shown in the table below. Depending on when you purchased the ECLIPSE Ni-E product, there may be products not yet available for use and products not listed that are already available for use. Contact your nearest Nikon representative for details.
  • Page 13 Chapter 1 Assembly Device Device Name Model Remarks Epi-fluorescence Epi-fluorescence attachment NI-FLEI D-FB Excitation Balancer attachable attachment HG Precentered Fiber Illuminator C-HGFI Mercury lamp Motorized HG precentered fiber illuminator C-HGFIE illuminator Can be attached to C-NA, D-ND6, NI-ND6-I, NI-N7-I, NI-ND6-E, Analyzer slider for DIC D-DA or NI-N7-E Nosepiece...

  • Page 14: Assembly Method

    Chapter 1 Assembly Assembly Method Introduction : Motorized Device Combination The available combinations are specified when installing the following four types of motorized device to Ni-E using contact arms. If any combination not allowed is used, an error message will be displayed when the power is turned on.

  • Page 15: Check Input Voltage

    Be sure to turn off the power to the microscope and peripheral devices before starting cable connection. Cable connections are described in each assembly step. Nikon recommends connecting cables at the end of the assembly (except when connecting a cable for the motorized universal condenser). See “25 Connect the motorized unit cable.”...

  • Page 16: Escape The Elevating Section

    ND connector on the connector box. NC B OU T Attaching the motorized ND filter wheel Attach the DIA motorized shutter (optional). The motorized shutter is attached by Nikon. Contact your nearest Nikon representative when the DIA motorized shutter needs to be mounted or removed.

  • Page 17: Attach The Lamp

    Chapter 1 Assembly Attach the lamp. Tool: Hex wrench (3 mm across flats) Insert the hex wrench and Lamp handling precautions loosen the fixing screw. Avoid touching the glass surface of the lamp with your bare hands. Fixing screw Loosen a lamphouse-cover fixing screw and lift up Lamphouse the cover to remove it.

  • Page 18: Attach The Contact Arm/Standard Arm

    Chapter 1 Assembly Attach the contact arm/standard arm. The attachment procedure is the same for standard arm and contact arm. A contact arm must be attached when using motorized accessories. The contact arm has a contact point. Remove the contact cover before mounting the motorized device on the arm.

  • Page 19: Attach The Epi-Fluorescence Cube Turret And Epi-Fluorescence Attachment (Required For Epi-Fluorescence Microscopy)

    Chapter 1 Assembly Attach the epi-fluorescence cube turret and epi-fluorescence attachment (required for epi-fluorescence microscopy). Tool: Hex driver (2 mm across flats) Hex wrench (3 mm across flats) First, connect the epi-fluorescence cube turret and Positioning Epi-fluorescence hole cube turret epi-fluorescence attachment and then attach on the Epi-fluorescence microscope arm.

  • Page 20: Attaching The Filter Cube

    Chapter 1 Assembly ■ Attaching the filter cube Precautions for attaching and removing the cube • Be sure to check that the light source is turned off before attaching or removing the cube. • Be sure to check that the power switch for control box A is turned off and then attach the cube by rotating the cube switchover turret.

  • Page 21: Replacing Excitation And Barrier Filters

    Orientation of the filter filter cube • The filter is two-faced. Be aware that the filter does not face a wrong side. Nikon filters have an arrow indication printed on the outer frame. Make sure that the arrow faces the direction of a dichroic mirror when attaching the filter.

  • Page 22: Attach The Motorized Barrier Filter Wheel (Optional)

    Chapter 1 Assembly Attach the motorized barrier filter wheel (optional). Tool: Hex driver (2 mm across flats) Hex driver (2.5 mm across flats, included with barrier filter wheel) Attach the motorized barrier filter wheel on the Barrier filter wheel Contact cover fixing screw epi-fluorescence cube turret and epi-fluorescence attachment.

  • Page 23: Attaching The Barrier Filter

    Chapter 1 Assembly ■ Attaching the barrier filter The wheel has one empty hole and seven filter holder attachment sections. The empty hole is address 1 and the barrier filter cannot be attached here. Attach the desired barrier filter (φ25/thickness 5 mm or less) in a wheel at address 2 to 8.

  • Page 24: Attach The Quadrocular Tilting Tube And Dsc Zooming Port For Quadrocular Tube

    Chapter 1 Assembly Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube. Tools: Hex driver (2 mm across flats) Hex driver (2.5 mm across flats, included with DSC zooming port) Hex wrench (3 mm across flats) Hex wrench (4 mm across flats) First connect the quadrocular tilting tube and DSC zooming port for quadrocular tube and then attach them on the microscope arm (if fluorescence cube turret,...

  • Page 25: Attach The Motorized Excitation Filter Wheel (Optional)

    Chapter 1 Assembly Attach the motorized excitation filter wheel (optional). Tool: Hex wrench (3 mm across flats) Connect the opening of the excitation filter wheel to the bayonet mount of the epi-fluorescence attachment. Press the excitation filter wheel supporting fixture against the rear of the epi-fluorescence attachment (location (2)) while pressing it against the bottom (location (1)) of the excitation filter wheel.

  • Page 26: Attaching The Excitation Filter

    Attach the EPI motorized shutter (optional). The motorized shutter is attached by Nikon. Contact your nearest Nikon representative when the EPI motorized shutter needs to be mounted or removed. Attach eyepieces. Make sure the notch on the eyepiece side and the...

  • Page 27: Attach The Substage

    Chapter 1 Assembly Attach the substage. Tools: Hex driver (2 mm across flats) Hex wrench (3 mm across flats) Place the groove of the substage on the reference guide bar in the elevating section of the main body. Move the substage to the left side, push it against Substage fixing screw the reference surface, and then tighten the...

  • Page 28: Attach The Stage

    Chapter 1 Assembly Attach the stage. 17.1 Rotatable ceramic coated stage Tool: Hex driver (2 mm across flats) Centering tool: provided ball-pointed hex drivers (x2) Sufficiently loosen the right and left centering screws (x2) on the back of the substage and the stage positioning screw on the front of the stage using a hex driver.
  • Page 29 5 6 1 0 0 1 This device complies with Part 15 of the FCC Rules.Operation is subject to the following two conditions: MODEL ECLIPSE Ni-E (1) this device may not cause harmful interference,and NIKON CORPORATION this device must accept any interference received, including interference 9 1 0 0 0 1 that may cause undesired operation.
  • Page 30 Chapter 1 Assembly [Fuse replacement method] WARNING • To prevent electric shock or malfunctions, always turn off the power of the motorized XY stage controller (press to the “O” position) and unplug the power cord from the wall outlet before replacing the fuse. •...

  • Page 31: Attach The Condenser

    Chapter 1 Assembly Blown Pull the blown fuse and remove it from the fuse fuse fuse holder. Place a new fuse in the fuse holder. 100-240V 50/60Hz Replacing the fuse Push in the fuse holder. AC IN 100-240V 2A 50/60Hz Fuse AC250V, T6.3AL Pushing the fuse holder...
  • Page 32 Chapter 1 Assembly Connect the condenser cable to the U-CON U-CON connector connector at the bottom of the contact arm. Condenser cable NC B Connecting the cable to the U-CON connector U-CON connector Contact arm bottom view Attach the provided cable cover to the condenser on the front side of the elevating section, and then tighten the fixing screws (x2) to fix it.

  • Page 33: [18.2] Manual Condenser (With The Condenser Holder)

    Engage the round dovetail of the condenser with the Attaching the condenser holder condenser holder so that the Nikon nameplate faces front and tighten the fixing screw of the condenser holder using a hex driver. Condenser fixing screw Securing the condenser ■...
  • Page 34 Chapter 1 Assembly Universal Condenser Structure (example: NI-CUD-E) Module U-CON connector replacement cover Condenser focus knob Condenser focusing unit Condenser lens PH module centering knob Tightening Pull the knob up horizontally when using the screw centering screw. Otherwise keep it folded down. Remove the cover.
  • Page 35 Chapter 1 Assembly [Procedure for attaching the DIC module on the condenser, dark-field module, and 2-4x auxiliary lens] Holes (5), (6), and (7) have their positioning pin and module fixing screw. • First check that the tip of the module fixing screw is not inside the hole. If it is inside, place that hole into the optical path, lift the PH module centering knob at right rear of the condenser, and turn the centering screw to loosen the module fixing screw.
  • Page 36 Chapter 1 Assembly Combinations of the DIC Slider on the Objective and the DIC Module on the Condenser (when NI-CUD-E/NI-CUD universal condenser is used) Standard combination Contrast-oriented Resolution-oriented Objective DIC module DIC slider DIC module DIC slider DIC module DIC slider (condenser) (objective) (condenser)

  • Page 37: Attaching The Dic Module To Dic Condenser

    Chapter 1 Assembly ■ Attaching the DIC module to DIC condenser D-CUO is provided with two sliders. Incorporate the oil condenser-specific DIC modules [D-C DIC N2 OIL] and [D-C DIC NR OIL] to each slider. Select the DIC module appropriate for the objective. (See the next table.) To support for particular purposes, modules for higher contrast or resolution are available.
  • Page 38 Chapter 1 Assembly Combinations of the DIC Slider on the Objective and the DIC Module on the Condenser (when D-CUO DIC Condenser Oil is used) Standard combination Resolution-oriented Objective DIC module DIC slider DIC module DIC slider (condenser) (objective) (condenser) (objective) Plan Fluor 10X Plan Apo 10XA (DIC allowed for eco...

  • Page 39: Attach The Nosepiece

    Chapter 1 Assembly Attach the nosepiece. Tool: Hex driver (2 mm across flats) Lift the nosepiece at a position slightly toward yourself than directly below the arm and attach while sliding it back. Continue sliding the nosepiece back until its front position is aligned with the front of the arm.

  • Page 40: Attach The Eyelevel Riser (Optional)

    Chapter 1 Assembly Attach the eyelevel riser (optional). Tool: Hex driver (2 mm across flats) Place the eyelevel riser on the arm and tighten the fixing screw on the front of the arm using a hex driver (2 mm across flats). Eyelevel riser fixing screw Eyelevel riser Fixing the eyelevel riser...

  • Page 41: Binocular Tube

    Chapter 1 Assembly Installing a camera head to the DSC 23.2 port for ergonomic binocular tube Tool: Hex driver (2 mm across flats) Screw the camera head into the C mount on the Camera cable connector DSC port. Remove the rear cover of the ergonomic tube and Camera head insert the DSC port.

  • Page 42: Replace The Nd Filter

    Chapter 1 Assembly Replace the ND filter (optional). Tool: Hex driver (2 mm across flats) The ND filter in the ND filter cassette of the Ni-E main Rubber cap body can be replaced as follows: Check that the power switch for control box A is OFF. Press all ND filter IN/OUT switches to the “IN”...

  • Page 43: Connect The Power Cord

    STAGE (Connect to ergo controller (Connect to MICROSCOPE for joystick controller) the motorized XY stage controller) MODEL ECLIPSE Ni-E DIA SHUTTER NIKON CORPORATION TOKYO, JAPAN 9 1 0 0 0 1 M A D E I N J A P A N This device complies with Part 15 of the FCC Rules.
  • Page 44 Chapter 1 Assembly...

  • Page 45: Troubleshooting

    If you detect problems that are not listed in the table or the problem still persists after measures are taken, turn off the device and contact your nearest Nikon representative.

  • Page 46: Optical System And Operation

    Chapter 2 Troubleshooting Optical System and Operation General Problem Cause Measure Dirt or dust rotates when the eyepiece is turned. The eyepiece is dirty. Clean the eyepiece. (→See Chapter 3 “2.1 Cleaning Lenses” in the “Assembly/Maintenance”.) Dirt or dust does not rotate when the eyepiece is turned.
  • Page 47 Chapter 2 Troubleshooting Problem Cause Measure Make sure the condenser is focused and The field diaphragm image is not focused centered. on the specimen surface. (See Chapter 3 “7 Focusing and Centering the (Condenser adjustment is incorrect.) Condenser” in the “Operation”.) No immersion oil is applied to the tip of an Apply our designated non-fluorescent oil-immersion objective.
  • Page 48 If the objective does not have a stopper, do protection of the objective is pushed in. not pull because the tip cannot be turned. In this case, contact your nearest Nikon representative. Screw the objective all the way in. Objective is attached incorrectly.
  • Page 49 Chapter 2 Troubleshooting Problem Cause Measure Attach it correctly and rotate it to the click stop The nosepiece is attached incorrectly or position. not rotated to the click stop position. (→See Chapter 1 “3 Assembly Method - 19 Attach the nosepiece” in the “Assembly/Maintenance”.) Position the specimen in place on the stage.

  • Page 50: Epi-Fluorescence Microscopy

    The objective or cover glass is dirty. (→See Chapter 3 “2.1 Cleaning Lenses” in the “Assembly/Maintenance”.) Use the non-fluorescent immersion oil designated by Nikon. The immersion oil is fluorescent. (→See Chapter 3 “16.9 Other Notes on Epi-fluorescence Microscopy” in the “Operation”.)

  • Page 51: Differential Interference Contrast Microscopy

    Chapter 2 Troubleshooting Differential Interference Contrast Microscopy Problem Cause Measure Rotate it to the click stop position. The universal condenser's turret is at the (→See Chapter 3 “9.3 Using the Universal intermediate position. Condenser (Dry) NI-CUD”.) Push in the slider until it reaches the limit. The DIC slider is at the intermediate (→See Chapter 3 “9.4 Using the D-CUO DIC position for an oil condenser.

  • Page 52: Phase Contrast Microscopy

    Chapter 2 Troubleshooting Problem Cause Measure Adjust the orientation of the optical system correctly. (→See Chapter 2 “5 Differential Interference The orientation of the polarizer is incorrect. Contrast Microscopy Procedure - 14 Adjust the orientation (vibration direction) of the polarizer and analyzer”...

  • Page 53: Electrical System

    Chapter 2 Troubleshooting Electrical System General ■ Power Problem Cause Measure The power cord is not connected, or is Connect the cable properly. There is no power even connected improperly. Or, the cable (→See Chapter 1 “3 Assembly Method - 25 Connect though the power switch between the control box and the main body the motorized unit cable”...
  • Page 54 Chapter 2 Troubleshooting ■ Controls/buzzer on the main body Problem Cause Measure Change the setting. (→See Chapter 3 “23 Operation on DS-L3” - “23.1 Buttons on the main Operation is disabled. Setting Up the Microscope - (3) Configuring the body do not respond. Button Functions - (3-1) Ni-E microscope button”...
  • Page 55 Chapter 2 Troubleshooting Problem Cause Measure XYZ speed interlock is Information for objectives is not Configure the objective information. ON, but the sensitivity of configured. (→See Chapter 3 “23 Operation on DS-L3” - “23.1 vertical stage movement (If the objective information is not Setting Up the Microscope - (1) Setting Optical does not switch configured, the sensitivity of vertical stage...
  • Page 56 Chapter 2 Troubleshooting Problem Cause Measure Turn the setting ON. (→See Chapter 3 “23 Operation on DS-L3” - “23.1 The interlock setting is OFF. The motorized universal Setting Up the Microscope - (4) Configuring the condenser's aperture Movement” in the “Operation”.) diaphragm does not Check motorized device combination and interlock when the...
  • Page 57 Chapter 2 Troubleshooting ■ Motorized ND filter Problem Cause Measure Connect the cable properly. Motorized nosepiece The motorized ND filter cable is not (→See Chapter 1 “3 Assembly Method - 25 Connect does not move at all. connected. the motorized unit cable” in the “Assembly/Maintenance”.) Turn the setting ON.
  • Page 58 Chapter 2 Troubleshooting ● Horizontal stage movement Problem Cause Measure Connect the stage properly. The motorized XY stage is not connected (→See Chapter 1 “3 Assembly Method - 17 Attach properly. the stage” in the “Assembly/Maintenance”.) Rotating the stage Press the operation button to release the knobs does not move specimen removal position.

  • Page 59: Epi-Fluorescence Microscopy

    - ■ Attaching the filter cube” in the “Assembly/Maintenance”.) The shutter does not The shutter does not shield light for some Contact your nearest Nikon representative for move. (A series of short reason. advice. beep sounds.) Configuration “OPEN”...
  • Page 60 Chapter 2 Troubleshooting...

  • Page 61: Maintenance And Storage

    Chapter Maintenance and Storage This chapter describes how to replace the halogen lamp, clean the product, restore microscope data to factory default, and transport/store the microscope.

  • Page 62: Replacing The Lamp

    Chapter 3 Maintenance and Storage Replacing the Lamp CAUTION  Beware of burns: Wait until the lamp and nearby parts have cooled (approximately 30 minutes) before replacing the lamp.  Beware of electrical shock: Turn off the power switch and unplug the power cord from the wall outlet. ...

  • Page 63: Cleaning

    Chapter 3 Maintenance and Storage Cleaning Clean and decontaminate the microscope and lenses in accordance with the following procedure. ■ Tools used for cleaning  Blower  Soft brush  Soft cotton cloth, lens tissue, gauze, etc.  Absolute alcohol (ethyl or methyl alcohol), medical alcohol ...

  • Page 64: Cleaning Immersion Oil

    If petroleum benzene is unavailable, use methyl alcohol alone. However, typically wipe three or four times because the detergency is weak. Decontaminating the Product For routine disinfection of this product, Nikon recommends using 70% medical alcohol. Use of organic solvents on plastic parts may result in discoloration. Cautions on disposal If contact occurs between a sample and this product, determine whether the sample is hazardous.

  • Page 65: Transportation (Using The Fastening Position Mode Switch)

    Chapter 3 Maintenance and Storage Transportation (Using the fastening position mode switch) Fastening position When transporting the Ni-E main body or any motorized mode switch accessories, the device must be fastened or moved into the (ON: fastening position mode, transportation position. OFF: normal mode) To fasten, press the fastening position mode switch at the rear of control box A to "ON"...

  • Page 66: Periodic Inspections (Charged)

    Chapter 3 Maintenance and Storage Periodic Inspections (Charged) To maintain the performance of this product, Nikon recommends periodic inspections (chargeable service). Contact your nearest Nikon representative for details.

  • Page 67: Specifications

    Chapter Specifications...

  • Page 68: Microscopy (Principles)

    ■ Intended user It is intended for the researchers, medical professional and those who work on experiments in the field of pathology and cytology. Performance Properties ■ Nikon Microscope ECLIPSE Ni-E + Control Box A Model ECLIPSE Ni-E + NI-CTLA Main body...
  • Page 69 Chapter 4 Specifications Tube Binocular tube (binocular section 100%) Trinocular tube F (Binocular : adapter = 100:0, 0:100) Trinocular tube T (Binocular : adapter = 100:0, 20:80, 0:100) Ergonomic tube (Binocular section 100%) (with DSC port mounted, binocular : DSC port = 50:50) Quadrocular tilting tube, motorized quadrocular tilting tube (Binocular : adapter = 100:0, 0:100) (with DSC port mounted, binocular : adapter : DSC port = 0:0:100)
  • Page 70 Chapter 4 Specifications ■ Motorized XY Stage + Motorized XY Stage Controller for Nikon Microscope Model NI-S-E + TI-S-CON Input rating voltage 100-240VAC, 50/60Hz, 2A Fuse rating 250V, 6.3A, time lag φ5.2 x 20 mm (Manufacturer: SOC Corp., Model: ET) •...

  • Page 71: Physical Properties

    Chapter 4 Specifications Physical Properties ■ Nikon Microscope ECLIPSE Ni-E + Control Box A Model ECLIPSE Ni-E + NI-CTLA Operating conditions Temperature: 0°C to +40°C Humidity: 60% RH max. (no condensation) Altitude: 2000 m max. Pollution degree: Degree 2 Installation:...
  • Page 72 Chapter 4 Specifications ■ Motorized XY Stage + Motorized XY Stage Controller for Nikon Microscope Model NI-S-E + TI-S-CON Operating conditions Temperature: 0°C to +40°C Humidity: 60% RH max. (no condensation) Altitude: 2000 m max. Pollution degree: Degree 2 Installation:...

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