Focus And Center The Condenser - Nikon Eclipse Ni-E Instruction Manual

13

Focus and center the condenser.

(1) Press the DIA field diaphragm button to close the
field diaphragm to a minimum and look into the
eyepiece. Focus on the field diaphragm image using
the condenser focus knob, and then adjust the
condenser centering screws to center the diaphragm
image within the field of view.
(2) With the objective vertical movement lever at the
center position, pull the sliding nosepiece and bring
the rear objective (40x or 60x attached) into the
optical path. (The condenser turret remains at the
[O] empty position.)
(3) Turn the objective vertical movement lever to the
right end, turn the parfocal correction knob on the
right side of the sliding nosepiece, and focus on the
sample. Turning the knob clockwise raises the
objective (focus position rises).
For information on the relationship between the
position of the objective vertical movement lever and
the position of the objective, see Chapter 3, "11.1
Objective Vertical Movement Lever (objective
switching, dipping)".
Cautions when using the parfocal
correction knobs
Before using the parfocal correction knobs, make sure
that the objective vertical movement lever is at the
right or left end. The parfocal correction knob on the
left side is for adjusting the front objective, and the
parfocal correction knob on the right side is for
adjusting the rear objective.
(4) Turn the condenser focus knob to focus on the field
diaphragm image. If the image is not at the center of
the field, turn the condenser centering screw so that
the image is positioned at the center.
(5) Press the DIA field diaphragm button and adjust the
field diaphragm image so that its size is close to the
field of view.
(See Chapter 3, "7 Focusing and Centering the
Condenser".)
14
Water-dip objectives (water immersion).
(1) Slowly move the objective vertical movement lever
to the right end, and then press it further right.
This lowers the objective about 1 mm, immersing the
tip of the objective in the liquid on the specimen.
(2) Stop pushing the lever and return it to right end.
The objective also returns to the original height.
(See Chapter 3, "11.1 Objective Vertical Movement Lever
(objective switching, dipping)".)
Chapter 2
Microscopy Operations
IR-DIC Microscopy Preparation
Procedure
1 2 3 4 5 6
Focus and Orientation Microscopy
7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
Bringing rear objective into optical path
Field diaphragm
image
Eyepiece
field ring
Focusing and centering the condenser
Objective vertical
movement lever
FN -S2 JAPA
N N
JAPA
FN -S2 N
N
Dipping objectives
20
A . S
Field diaphragm
image
Eyepiece
field ring
Parfocal adjustment knob
(for rear objective)
10x
40x