Turn Off The Mercury Lamp - Nikon Eclipse Ni-E Instruction Manual

Viewing the sample
12
View the sample.
To observe another part of the sample, rotate the stage
knobs to find the position to observe. If the sample is not
in focus, rotate the focus knobs to adjust the focus.
To observe under a different magnification, repeat steps 6
and later with another objective.
Diascopic image in fluorescence
observation
For fluorescence observations, press the
microscope's dia-illumination ON/OFF switch to
"OFF" to make the diascopic image disappear.
Bright ambient lights will make it difficult to view
the image. Nikon recommends keeping the room
dark during fluorescence observations.
FL/DIC analyzer slider NI-FA/dummy
slider
For epi-fluorescence microscopy, the FL/DIC
analyzer slider inserted in the epi-fluorescence
cube turret is not used. If the analyzer slider is
inserted, pull it out to the first click-stop position
and remove the analyzer from the optical path. If a
dummy slider is inserted instead of the analyzer
slider, be sure not to insert all the way, but to pull it
out by one position. The periphery of field of view
may be dark if it is inserted all the way in.
13

Turn off the mercury lamp.

See your illuminator's manual for details.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
36
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
IN TE NS
ILI GH T
C- H G
FI E
LA MP
RU N
TI ME
hr s.
PO WE
R
Turning off the mercury lamp
A . S
POWER indicator
(green) OFF
Power switch OFF