Adjust The Aperture Diaphragm - Nikon Eclipse Ni-E Instruction Manual

15

Adjust the aperture diaphragm.

Turn the condenser aperture diaphragm lever to adjust the
aperture. The aperture should be adjusted to about 70 to
80% of the numerical aperture of the objective. (See
Chapter 3, "8 Adjusting the Aperture Diaphragm".)
Appropriate size of the aperture
diaphragm
Normally, the appropriate size of the aperture
diaphragm is 70 to 80% of the numerical aperture
of the objective. Since an excessively small
aperture diaphragm opening will degrade the
image resolution, Nikon does not recommend
setting the aperture diaphragm to less than 60%
of the numerical aperture of the objective.
Aperture diaphragm adjustment timing
Be sure to adjust the aperture diaphragm each
time you change the objective.
16
Focus on the sample.
Focus on the sample by rotating the focus knob. (See
Chapter 3, "4.1 Proper Focusing Procedure".)
Chapter 2
Microscopy Operations
IR-DIC Microscopy Preparation
Procedure
1 2 3 4 5 6
Focus and Orientation Microscopy
7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
Adjusting aperture diaphragm
Pupil of
objective
Right size of the aperture diaphragm
Focusing using the focus knob
_ _ _ _ _ _ _
O P E N _ _ _ _ _ _ _ _ _ _ _ _
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Aperture
diaphragm image
70 to 80
100
A . S
_ Z : _ _ _ _ _ 1 . 2 3 4 u m
F S 3 0 . 0 m m
Z-axis coordinate