Nikon Eclipse Ni-E Instruction Manual page 99

NCB filter
The Ni-E's filter cassette is equipped with an NCB filter for
color temperature conversion.
(6) Align the specimen.
(7) Readjust the focus onto the target.
(8) Adjust the image brightness using the camera exposure compensation function.
(9) Check the image using the DS-L3 [FREEZE] button.
(10) If the image is acceptable, press the CAPTURE button to save the image.
(For details, see the instruction manual provided with the camera.)
Number of images captured
Seriography is not available because the CAPTURE
button is designed to capture only one image.
ZOOM
Attaching a camera to the zooming port allows to zoom in an image.
■ Motorized DSC Zooming Port
The final magnification of the motorized DSC zooming port
can be from 0.6x up to 2.0x.
Use the CW/CCW button of the ergo controller for a zoom
operation. A zoom magnification is displayed in 0.1x
increments. See the Ni-E (Focusing Stage System)
instruction manual for details of the ergo controller.
Operation on DS-L3 (See "20.2 Microscope Control".)
Limited zoom range
Note that the zoom range may be limited depending on
capture conditions or type of the camera when using a DS
camera made by other manufacturers.
Limb darkening at the zoom port
• When performing bright field microscopy with 2-layer
epi-fluorescence cube turret, the edge of the field of
view may be dark at low zoom magnification.
• When performing epi-fluorescence microscopy with
2-layer epi-fluorescence cube turret, the edge of the
field of view may be dark at low zoom magnification.
Manual DSC zoom port
See the Ni-E (Focusing Stage System) instruction manual
for details of the manual DSC zoom port.
Chapter 3
Individual Operations
85
Z Speed XY Speed
FL cube OBJ
Fl shutter B/F/R EPI all
Zoom operation
P A _ _ 2 x _ _ _ = X : _ _ _ = = 0 . 0 0 0 u m
Z M : 1 . 0 x _ _ _ Y : _ _ _ _ _ 0 . 0 0 0 u m