Using The Condenser - Nikon Eclipse Ni-E Instruction Manual

9

Using the Condenser

The focusing nosepiece system uses the FN-C LWD condenser appropriate for dia-illumination bright-field microscopy
(objective 4x to 100x), dia-illumination differential interference contrast microscopy (infrared ray range, visible range) and
diagonal illumination microscopy.
■ Optical module
The turret of the LWD condenser has one empty hole and three holes for attaching optical modules. Attach the optical
module suitable for each microscopy to the turret. See Chapter 1 "3 Assembly Method - 9 Attach the condenser -
■ Attaching the optical module to the condenser turret" in the "Assembly/Maintenance" instruction manual for details on
the attachment procedure.)
• DIC module (required for differential interference contrast microscopy)
DIC module includes [D-C DIC N1 DRY], [D-C DIC N2 DRY], and [D-C DIC NR DRY].
Select the DIC module appropriate for the microscopy method and objective. If the combination is not correct,
differential interference contrast image cannot be obtained or the contrast decreases significantly. Depending on the
purpose of observation, dedicated modules for higher contrast or resolution are available. Note, however, that in
principle the contrast contradicts the resolution of the differential interference contrast image (the higher the
contrast, the lower the resolution). (See the table in Chapter 1, "3 Assembly Method - 9 Attach the condenser -
Combination of DIC Slider on the Objective and DIC Module on the Condenser (when using the FN-C LWD
condenser)" in the "Assembly / Maintenance" instruction manual.)
• 4x auxiliary lens (required for low magnification bright-field microscopy)
When performing 4x bright-field microscopy with a LWD condenser, attach a 4x auxiliary lens to the mount position
of DIC module N1. 4x is not indicated on the turret. Remove it when viewing with the objectives greater than 4x
magnification.
• Diagonal illumination diaphragm
Attach a diagonal illumination diaphragm to the turret in order to increase the contrast when observing a sample.
Using diagonal illumination, contrast can be attained so that even a nearly transparent sample can be observed.
You can also adjust the shadow on the sample by turning the diagonal illumination diaphragm rotation ring which
rotates the diaphragm around the optical axis.
■ Switching the optical module (switching the turret)
Rotate the condenser turret manually to switch. It can be
turned either clockwise or counterclockwise. The attached
optical module is indicated on the turret side and the optical
module indicated at the front of the turret is in the optical
path.
Make sure the turret is stopped at a click-stop position when
rotating.
Display [N1], [N2]: DIC module ([N1] when a 4x auxiliary
lens is attached.)
Display [OBL]: Diaphragm for the diagonal illumination
[O]: Empty hole
Chapter 3
Individual Operations
Optical module
indicator
in optical path
59
Turret
Aperture diaphragm
Diagonal illumination
lever
diaphragm rotating ring
LWD condenser