Nikon Eclipse Ni-E Instruction Manual page 52

Switching from IR-DIC (infrared ray differential interference contrast) microscopy to
epi-fluorescence microscopy
Epi-fluorescence and differential interference contrast methods can be used concurrently to cover the shortcomings
of each. For example, when locating the target, differential interference contrast microscopy can be used instead of
epi-fluorescence microscopy which tends to cause decoloration of the specimen.
This section provides tips for switching from IR-DIC (infrared ray differential interference contrast) microscopy to
epi-fluorescence microscopy when devices required for epi-fluorescence microcopy and IR-DIC (infrared ray
differential interference contrast) microscopy are attached. Precautions on concurrent use of
epi-fluorescence/differential interference contrast microscopy in the visible light range are also noted. Also see "3
IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure".
(1) When turning on the power switch at the beginning
of "3 IR-DIC (Infrared Ray Differential Interference
Contrast) Microscopy Procedure", keep the power of
the HG precentered fiber illuminator for
epi-fluorescence illumination OFF.
Check that the epi-fluorescence illumination path
shutter is closed.
The shutter is closed when the [FL SHUTTER]
button is lit.
(2) All optical elements required for differential
interference contrast microscopy are once removed
from optical path in procedures 6 and 7 of "3 IR-DIC
(Infrared Ray Differential Interference Contrast)
Microscopy Procedure".
Focus and adjust the optical system by IR-DIC
(infrared ray differential interference contrast)
microscopy in this state.
(3) Adjust the orientation of the polarizer and analyzer
by procedure 19 of "3 IR-DIC (Infrared Ray
Differential Interference Contrast) Microscopy
Procedure".
(4) When IR-DIC (infrared ray differential interference
contrast) microscopy is finished, go to
epi-fluorescence microscopy.
• Press the dia-illumination ON/OFF switch to turn
off the lamp and make the diascopic image
disappear.
• Rotate the epi-fluorescence cube turret to bring
the desired filter cube into the optical path.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
38
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
Lit
Closing shutter
Empty
2
3
4
5
position
Remove the
DIC slider
Empty
position
Removing the DIC optical element from the optical
path
Turning off dial-illumination lamp
Bringing the filter cube into the optical path
ND ND NCB
8 32 11
OUT
IN
A. ST
P
F. STO
6
Remove the
FL/DIC
analyzer
slider.
FN- S2N JAPAN
ND
8
OU T
IN
ND ND NCB
8 32 11
OUT
IN