Focusing On The Sample (Vertical Objective Movement); Proper Focusing Procedure - Nikon Eclipse Ni-E Instruction Manual

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Focusing on the Sample (Vertical Objective Movement)

Focus adjustment is performed using the focus knobs on the Ni-E main body, ergo controller, or focus knob on joystick.
The following instructions assume the use of the focus knobs on the main body. See the Ni-E (Focusing Stage System)
instruction manual for information on using the ergo or joystick controller.
"Resetting the Z-axis coordinate," "Refocusing," and other useful functions are available for focus adjustment.

Proper Focusing Procedure

4.1
Note on controlling the focus knobs
When operating the focus knobs on the main body, avoid the
following action, which can cause equipment malfunctions.
Rotating the right and left focus knobs in opposite directions.
Using an objective of high magnification may cause the
objective to be pushed against the sample, damaging the
objective. Focus according to the following procedure:
(1) Bring the 10x or 4x objective into the optical path, and turn
the coarse focus knob to lower the objective to the lower
limit.
(2) Direct 100% of the light to the binocular section.
(3) Adjust the brightness of dia-illumination.
(4) Look into the eyepiece and turn the coarse focus knob to
raise the objective and focus on the sample.
(5) Turn the fine focus knob to further adjust the focus.
Tips for focusing
• When lowering the objective with the coarse focus knob, move your eyes away from the eyepiece and operate the
microscope while looking at the microscope from the side.
• When working with the coarse focus knob while looking into the eyepiece, you should only turn the knob in the
direction for raising the objective.
• First use an objective of low magnification to adjust the focus, and then switch to an objective of higher
magnification.
• Since the 10x or 4x objective has a wider operating distance, the sample will not touch the tip of the objective
provided the sample has standard thickness.
Chapter 3
Individual Operations
50
A . S
Do not turn the focus knob!
A . S
Using the focus knob to focus
P A _ _ 2 x _ _ _ _ Z : _ _ _ _ _ 1 . 2 3 4 u m
U V - 2 B C _ Z M : 1 0 x _ N D :
' '
The value increases when the objective is
raised, and decreases when it is lowered.
_ 0 . 0 %