Nikon Eclipse Ni-E Instruction Manual page 48

Focusing and Optical System Adjustment
8
Focus on the sample.
Focus on the sample by rotating the focus knob. (See
Chapter 3, "4.1 Proper Focusing Procedure".)
Z-RESET
The Z-axis coordinate value can be reset to zero.
This is useful when you wish to use the current
position as the reference position in adjusting the
focus. (See Chapter 3, "4.3 Resetting the Z-axis
Coordinate".)
Refocusing
This operation moves the Z-axis position to the
retracting position or returns it to the original
position. (See Chapter 3, "4.4 Refocusing".)
Note on controlling the focus knobs
Avoid the following action, which can cause equipment
malfunction.
• Rotating the right and left focus knobs in opposite
directions.
Center the field diaphragm of the
9
epi-fluorescence attachment.
(1) Use the field diaphragm lever on the
epi-fluorescence attachment to close the field
diaphragm, and then move the field diaphragm
image to the center of the field of view by turning the
field diaphragm centering screw using a hex driver
(2 mm across flats).
(2) Open the field diaphragm to fit the field of view, and
once again, turn the centering screw to move the
field diaphragm image to the center of the field of
view.
Changing magnification
While it is not absolutely essential to center the
field diaphragm each time you change the
objective, you should still perform a quick check
and make adjustments as necessary.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
34
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
Focusing using the focus knob
P F _ 4 0 x _ _ _ _ Z :
U V - 2 B O _ _ _ _
'
Do not turn the focus knobs in opposite direction!
F. ST OP
Field diaphragm lever
Field diaphragm
image
Eyepiece
field ring
Centering the field diaphragm
A . S
_ _ _ _ _ 7 . 1 2 3 u m
N D _ 1 O _ _ E P I : O
'
Z-axis coordinate
A . S
A. ST OP
Field diaphragm
image
Eyepiece
field ring