Focus And Orientation; Bring The 4X Or 10X Objective Into The Optical Path; Place A Sample On The Stage, And Move The Stage To Bring The Target Into View - Nikon Eclipse Ni-E Instruction Manual

IR-DIC Microscopy

Focus and orientation

8

Bring the 4x or 10x objective into the optical path.

(1) Move the objective vertical movement lever to the
center. (to temporarily retract the objective upward)
(2) Push the sliding nosepiece toward the rear, and
bring the low magnification objective (4x or 10x)
attached to the front into the optical path. (See
Chapter 3, "11 Operating the Nosepiece (objective
operation".)
Cautions on switching objectives
Be sure to move the objective vertical movement lever
to the center before switching objectives (between
front and rear).
Objectives cannot be switched when the objective
vertical movement lever is at the right or left end.
Undue force may result in malfunction.
When using a 4x objective
When using a 4x objective, attach the 4x auxiliary
lens to the LWD condenser turret and bring it into
the optical path. When using an objective with
magnification higher than 4x, remove the 4x
auxiliary lens from the optical path.
9

Place a sample on the stage, and move the stage to bring the target into view.

(1) Set the sample at the center of the stage.
(2) Rotate the stage X/Y knobs to move the stage and
bring the target of the sample into the optical path.
(See Chapter 3, "5 Bringing the Target into the
Optical Path (Horizontal Stage Movement)".)
Motorized horizontal stage movement
When using a motorized XY stage, use the ergo or
joystick controller to control the horizontal stage
movement. (See the Ni-E (Focusing Stage
System) instruction manual for details on the ergo
controller or joystick controller.)
Chapter 2
Microscopy Operations
Preparation
Procedure
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
17
Focus and Orientation Microscopy
FN -S2
(1)
(2)
Bringing the 4x or 10x objective into the optical
path
Setting sample
Y direction
Stage X knob
Stage knob operation and stage moving direction
JAPA N
N
40x
10x
Select 4x or 10x.
JAPA
FN -S2 N
N
40x
10x
X direction
NC B
ND
ND 11
32
8
OU T
IN
Stage Y knob