Epi-Fluorescence Microscopy; Epi-Fluorescence Cube Turret Operation (Switching Excitation Methods) - Nikon Eclipse Ni-E Instruction Manual

17

Epi-fluorescence Microscopy

An epi-fluorescence microscopy used for the observation of a fluorescence image requires a brighter illuminator including
such an optical element as a filter or mercury lamp.
Ni-E uses an epi-fluorescence attachment with a fluorescence cube turret to which a filter cube is attached (motorized,
intelligent, or manual), HG precentered fiber illuminator using a mercury lamp as an illuminator (motorized or manual), ND
filters, an aperture diaphragm and a field diaphragm.
WARNING
The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling
because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at
the beginning of this instruction manual.

Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)

17.1
The epi-fluorescence cube turret has six slots. Attach the epi-fluorescence cube to a slot and bring it into the optical path.
For bright-field microscopy, be sure to set Address 1 to empty [OPEN] and bring it into the optical path.
DIC analyzer cube
Attach a DIC analyzer cube to the epi-fluorescence cube turret to perform differential interference contrast microscopy
with the Ni-E focusing nosepiece system. (See "16 Differential Interference Contrast Microscopy".)
Motorized epi-fluorescence cube turret operation
Use a FL CUBE CW/CCW button to switch the epi-fluorescence
cube turret. The turret rotates to the adjacent address each time
the button is pressed.
The attach information on the filter cube has not been set as
default.
Information on the filter cube on the optical path is displayed on
the display panel after setting the attach information on the filter
cube to the epi-fluorescence cube turret before switching.
In addition, the front slot cover for a filter cube replacement has
a window for inserting a filter cube nameplate. It is also useful if
you keep inserting the nameplate into the window at the address
to which the filter cube was attached.
The setting of the attach information is performed from the
DS-L3. (See "20 Operation on DS-L3" - "20.1 Setting Up the
Microscope - (1) Setting Optical Elements Installation
Information" for details.)
See Chapter 1, "3 Assembly Method - 14 Attach the
epi-fluorescence cube turret and epi-fluorescence attachment" -
■ Attach the filter cube/analyzer cube" in the
"Assembly/Maintenance" instruction manual for information on
how to attach the filter cube to the turret.
Operation on DS-L3 (See "20.2 Microscope Control".)
Chapter 3
Individual Operations
69
Switching of the epi-fluorescence cube turret
P A _ _ 2 x _ _ _ _ Z : _ _ _ _ _
U V - 2 B C _ _ = _ N D _
'
2
3
4
5
6
FN -S2 JAPA
N N
Filter cube nameplate
window
Inserting the nameplate into the address window
ND ND NCB
8 32 11
OUT
IN
0 . 0 0 0 u m
1 C _ _ E P I : C
'