Operation Flowchart - Nikon Eclipse Ni-E Instruction Manual

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Operation Flowchart

The microscopy operation flow is shown below.
See the procedure for each microscopy operation in the next and subsequent sections for details.
IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure
1 Turn on the power.
2 Turn on the dia-illumination lamp.
3 Adjust optical path in tube to direct 100% of light to binocular.
4 Lower the condenser slightly from the uppermost position.
5 Fully open the field diaphragm and aperture diaphragm.
6 Set the condenser turret to [O] position (empty: bright-field).
Move the epi-fluorescence cube turret to empty position, and remove
the DC slider (objective side) and polarizer from the optical path.
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8 Bring the 4x or 10x objective into the optical path.
9 Place a sample on the stage, and move the stage to bring the target into view.
10 10 Focus on the sample.
11 11 Perform diopter adjustment.
12 12 Adjust the interpupillary distance.
13 13 Focus and center the condenser.
14 14 Water-dip objectives (water immersion).
15 15 Adjust the aperture diaphragm.
16 16 Focus on the sample.
17 17 Adjust the field diaphragm.
18 18 Adjust the brightness.
19 19 Adjust the orientation (vibration direction) of the polarizer and analyzer.
20 20 Attach the DIC slider (objective side) to the slider nosepiece.
21 21 Bring the DIC module (condenser side) into the optical path.
22 22 Observe a sample using a microscope.
23 23 Turn off the power.
Diagonal illumination
Chapter 2
Microscopy Operations
Preparation Preparation
Focus and orientation Focus and orientation
Viewing the sample Viewing the sample
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