Diagonal Illumination - Nikon Eclipse Ni-E Instruction Manual

IR-DIC Microscopy

Diagonal illumination

Using diagonal illumination, contrast can be applied to almost transparent samples thus making it possible to
observe them. In addition, since the slit of the diaphragm for diagonal illumination can be rotated centering around
the optical axis, you can adjust the direction of the shadow on the sample.
Bring the diaphragm for diagonal
1
illumination into the optical path.
Attach the diaphragm for diagonal illumination to the turret
on the condenser in advance. (See Chapter 1
"3 Assembly Method - 9 Attach the condenser -
■ Attaching the optical module to the condenser turret" in
"Assembly/Maintenance".)
Turn the turret and face the display [OBL] indication to the
front. The diagonal illumination diaphragm enters the
optical path in this state.
2
Adjust the direction of the illumination.
Rotate the rotation ring of the diaphragm for diagonal
illumination on the condenser turret to adjust the direction
of the illumination.
Chapter 2
Microscopy Operations
Preparation
Procedure
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
28
Focus and Orientation Microscopy
[OBL]: diaphragm for
the diagonal illumination
Bringing the diagonal illumination diaphragm into
the optical path, and adjusting the direction of the
illumination
Diagonal illumination
diaphragm rotation ring