Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual page 143

Setting
Size calling method
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
b. Enter a name and edit settings as needed (see "Fragment/HID analysis
settings (size calling)" on page 126).
c. As needed, touch View or Delete (user-created settings only).
3. Touch Done.
Edit sequencing settings
Factory-installed items cannot be edited or deleted. To create a new item from a
factory-installed item, copy, edit, then save the new item.
Analysis settings are accessible only to the user who creates them.
1. Access the Analysis settings screen:
From
Plate properties tab Touch More options4Analysis settings, then select the
Home screen
2. To create new analysis settings:
a. Touch the default analysis settings or user-created analysis settings, then
touch Copy.
b. Enter a name and edit settings as needed (see "Sequencing settings (base
calling)" on page 129).
c. As needed, touch View or Delete (user-created settings only).
3. Touch Done.
Fragment/HID analysis settings (size calling)
• Local Southern—
relationship between fragment length and electrophoretic mobility.
• Global Southern—Compensates for standard fragments with anomalous
electrophoretic mobility (similar to least squares methods).
• 2nd LSQ (2nd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• 3rd LSQ (3rd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• Cubic Spline Interpolation—Forces the sizing curve through all the known points
of the selected size standard.
Chapter 10 Manage the software (instrument)
settings to assign to the plate setup.
Touch Settings4Run settings 4Analysis settings, then
manage the list of settings that you can select from during
plate set up.
Description
(default) Determines the fragment sizes using the reciprocal
Manage run settings (instrument)
Action
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