Perform A Custom Dye Calibration - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

Perform a custom
dye calibration
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Condition number
A Condition Number indicates the amount of overlap between the dye peaks in the
fluorescence emission spectra of the dyes in the dye set.
If there is no overlap in a dye set, the Condition Number is 1.0 (ideal conditions), the
lowest possible value. The condition number increases with increasing peak overlap.
The ranges that the software uses to determine if a capillary passes or fails are:
Dye Set
AnyDye
D
E
E5
F
G5
J6
J6-T
Z
Add a custom dye set to the software based on a system dye
1. In the home screen, touch
Calibration4Dye calibration.
2. Touch Custom Dye.
3. Touch Add.
Appendix C Dye calibration and install standard checks
Quality Value Minimum
0.8 (default)
0.8
0.95
0.95
0.95
0.95
0.95
0.95
0.95
Settings4Maintenance and Service4
C
Calibrate dyes
Condition Number
Maximum
20.0 (default)
8.5
5.5
6.0
8.5
13.5
8.0
8.0
5.5
197