Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual page 141

Run module
LongSeq
LongSeq_BDX
[1]
CRL was determined using the Long Read Sequencing standard. A minimum of 90% of analyzed sequences with an average QV ≥QV threshold
were observed.
Table 8 Fragment analysis run modules
Run module
SNaPshot
FragAnalysis
[2]
LongFragAnalysis
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 or
GS1200 LIZ size standard sample sized with a third order fit) is ≥1. The table shows the resolution range in ≥90% of samples.
[2]
Load a maximum of 48 samples per plate if you use a long run module.
Table 9 HID run modules
Run module
HIDAnalysis
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 size
standard sample sized with a third order fit) is ≥1.
[2]
The GeneScan ™ 500 LIZ ™ Size Standard was not included in the HID validation of the instrument.
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Contiguous read
[1]
length (CRL)
≥800
IMPORTANT! Use BDX run modules only if you prepare samples with BigDye
™
XTerminator Purification Kit. Use non-BDX run modules for samples purified with
other methods.
Resolution Approximate
range run time
40–120 bp 25 minutes
[1]
60–460 bp 45 minutes
[1]
60–600 bp < 2 hours
Resolution Approximate
range run time
[1]
60–470 bp 39 minutes
Note: The following size standards have not been validated for use with the
instrument. A default size standard definition is not provided in the software.
·
™ ™
GeneScan 350 ROX
·
™
GeneScan 400HD ROX
Chapter 10 Manage the software (instrument)
QV threshold
QV20
Sizing precision
40–120: <0.5
60–460: <0.15
60–460: <0.15
461–600: <0.3
601–800: >0.45
Sizing precision
60–470: <0.15
Size Standard
™
dye Size Standard
Manage run settings (instrument)
Approximate run
time
~ 2 hours
Compatible size
standards
™
GeneScan 120 LIZ
Standard
All except GeneScan
™
1200 LIZ Size Standard
• GeneScan ™
Size Standard v2.0
™
• GeneScan
LIZ ™ Size Standard
Compatible size standards
• GeneScan ™ 500 LIZ ™
[2]
Standard
• GeneScan ™ 600 LIZ ™
Standard v2.0
10
™
Size
™
600 LIZ ™
1200
Size
Size
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