Check Size Standard Quality - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

A
Appendix A Troubleshooting

Check size standard quality

Quality alert
The number of size standard peaks detected is
less than what is defined in the size standard.
The analysis range is too small. Correct the
analysis range in analysis settings and re-
analyze in secondary analysis software or re-
inject sample.
Table 14 Basecalling quality alerts
Quality alert
Basecalling failed due to poor quality
data.

Check size standard quality

172
Size standard definition
includes peaks that are not
present in the sample. Example:
Sample peaks are detected up
to 500 bp, but the size standard
definition includes peak sizes
that are >500 bp.
Various causes.
Description
Poor quality data is present.
1. Touch Settings4Run history.
2. Touch a plate name, then touch View.
3. Touch a sample file name, then touch View.
If the data triggered any quality alerts, a QC alerts screen is displayed.
4. Touch View data to display the trace.
Description
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Action
Use or create a size standard
definition with the appropriate
number of peaks and peak
sizes.
• Analyze the data in a
secondary analysis
software with a corrected
analysis range.
• Re-inject the sample.
Action
• Re-inject the sample.
• If the problem persists, prepare
fresh sample.
• Troubleshoot upstream PCR and
sequencing steps.