Workflow: Hid Analysis; Hid Install Run - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

Workflow: HID analysis

HID install run

Item
Components of the install check
reaction
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
Prepare the instrument
Prepare the samples
Create a plate setup on the instrument
Create or import a plate setup
Enter plate properties
Assign wells: run module, size standard, dye set, and kit
Assign wells: sample name, sample type, and custom fields
Start and monitor a run
On the instrument: Load the plate or the tube assembly
Select a plate setup and start a run
Monitor a run from the instrument
View and analyze results
"View results on the instrument" on page 111
Export results from the instrument (sample data files and QC reports)
Analyze data in a secondary analysis software application
• GeneScan ™ 600 LIZ
• Hi‑Di ™ Formamide (Cat. No. 4311320 or 4440753)
• GlobalFiler ™ Allelic Ladder from one of the following installation kits:
– SeqStudio ™ HID and Sequencing Installation Kit (Cat. No. A46180)
– SeqStudio ™ HID Installation Kit (Cat. No. A46182)
Appendix D Create and run an HID plate
Get started
(page 39)
(page 37)
(page 81)
(page 82)
(page 84)
(page 86)
(page 92)
(page 93)
(page 105)
(page 121)
Description
™ Size Standard v2.0 (Cat. No. 4408399)
D

Workflow: HID analysis

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