View The Raw Data And The Ept Plot; Data Quality Alerts; Sizecalling And Basecalling Quality Alerts - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

View the raw data and the EPT plot

Data quality alerts

Quality alert
Offscale peaks. Adjust the injection
parameters and/or the sample
concentration.
No sample was detected.

Sizecalling and basecalling quality alerts

Table 13 Sizecalling quality alerts
Quality alert
Sizing quality value is low due to poor size
standard peak quality. Peak height uniformity is
low or the fitting quality in sizing is poor.
Sizecaller found broad peak(s) in the size
standard peak(s).
Sizing quality value is in the intermediate range;
check size standard data quality.
SeqStudio Genetic Analyzer Instrument and Software User Guide
™
The EPT view (ElectroPhoresis Telemetry) shows instrument data conditions
(currents, temperatures, electrophoresis voltage) as a function of time.
When a run is complete, in the home screen:
1. Touch Results.
2. Touch List view.
3. Touch an injection group.
4. Touch a sample file name.
If the data triggered any quality alerts, a QC alerts screen is displayed.
Click View data to display the trace for the sample.
5. Touch
or to scroll to the raw data or EPT Plot.
At least 10 scans have saturated the
CCD camera.
Poor signal‑to‑noise ratio with low
signal detected.
Low resolution or poor quality
data is present.
Description
Description
Appendix A Troubleshooting

View the raw data and the EPT plot

Action
• Reduce the injection voltage or
time.
• Dilute the sample.
• Verify that the sample volume
follows recommendations in the
user manual.
• Troubleshoot upstream PCR and
sequencing steps.
Action
• Re-inject the sample.
• If the problem persists,
check the sample quality.
A
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