C
Appendix C Dye calibration and install standard checks
Perform an install run
Export the install
run report
208
HID analysis install run pass/fail criteria
# Allele peaks
# Size standard peaks
Minimum peak height
Standard deviation of the observed allele fragment sizes
for all 4 capillaries
Sequence analysis install run pass/fail criteria
Result
QV30 CRL (contiguous read
length)
Note: The contiguous read
length passing criteria for
install checks is an
uninterrupted segment of
bases with an average Quality
Value (QV) of 30.
The remaining results on the screen are for information only.
1. Touch
Settings4Maintenance and Service4Install run4Install run history.
2. Touch the install run of interest.
3. Touch Export report.
4. Select a destination for the report.
5. Touch OK.
Result
Acceptance criteria
BigDye
™
Terminator v3.1
Capillaries with QV30 CRL ≥500 bp pass.
BigDye
™
Terminator v1.1
Capillaries with a QV30 CRL ≥500 bp pass.
SeqStudio
Genetic Analyzer Instrument and Software User Guide
™
Acceptance criteria
343 for all four capillaries
26 for all four capillaries
>400 RFU
<0.15 base pairs (bp)