Edit Injection Parameters And Re-Inject Samples; Export A Report (Qc Report) - Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer User Manual

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8
Chapter 8 View and analyze results
View results on the instrument
Quality alert
The analysis range is too small. Correct the
analysis range in analysis settings and re-
analyze in secondary analysis software or re-
inject sample.
Table 6 Basecalling quality alerts
Quality alert
Basecalling failed due to poor quality
data.
Edit injection
parameters and
re-inject samples
Export a report
(QC report)
120
Various causes.
Poor quality data is present.
You can edit injection parameters and re-inject samples during a run or after a run is
complete.
1. Access Injection options.
• During a run—Touch
• After a run—Touch Results.
2. Touch an injection group, then configure the injection list:
• Touch and drag an injection group to a new location in the injection list.
• Touch Inject first—Moves the selected injection group to the top of the
injection list.
• Touch Edit and re-inject—Adds replicates or re-injections to the injection list.
You can also modify Run module, Injection time, Injection voltage, Run
time, or Run voltage for these injections.
3. Touch Done.
Note: The changes are not applied until you touch Done.
This function allows you to export a QC report for the current plate. To export a QC
report for a previously run plate, export a run history (see "Export results from the
instrument (sample data files and QC reports)" on page 121).
When a run is complete, in the home screen:
1. Touch Results.
2. Touch Export report.
3. Select a storage location.
4. Navigate to, then select a location, then touch Export.
Description
Description
Actions4Edit plate.
SeqStudio
Genetic Analyzer Instrument and Software User Guide
Action
• Analyze the data in a
secondary analysis
software with a corrected
analysis range.
• Re-inject the sample.
Action
• Re-inject the sample.
• If the problem persists, prepare
fresh sample.
• Troubleshoot upstream PCR and
sequencing steps.

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