Abbott i-STAT 1 System Manual page 483

Specimen Collection and Preparation
The i-STAT ACT test can be performed using venous or arterial samples.
Kaolin
Venipunctures and Arterial Punctures
• Collection technique resulting in good blood flow must be used.
The sample for testing should be drawn into a plastic collection device (either a plastic
•
syringe or plastic evacuated tube).
The collection device cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
•
• The collection device cannot contain clot activators or serum separators.
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is required, a fresh sample must be obtained.
Note: Some experts recommend drawing and discarding a sample of at least 1 mL prior to drawing
sample for coagulation testing.
Indwelling line
• Fluid drip through the line must be discontinued.
• If blood must be drawn from an indwelling line, possible heparin contamination and specimen dilution
should be considered. The line should be flushed with 5 mL of saline and the first 5 mL of blood
or six dead space volumes should be discarded.
• Withdraw the sample for testing into a fresh plastic syringe.
• The collection syringe cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is needed, draw a fresh sample.
Extracorporeal line
• Flush the extracorporeal blood access line by withdrawing 5 mL of blood into a syringe and
discard the syringe.
• Withdraw the sample for testing into a fresh plastic syringe.
The collection syringe cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
•
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is needed, draw a fresh sample.
]References
1. Hattersly, P. Activated coagulation time of whole blood. Journal of the American Medical Association
136:436-440, 1966.
2. CLSI. Method Comparison and Bias Estimation Using Patient Samples; Approved Guideline. CLSI
document EP9-A (ISBN 1-56238-283-7). CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania
19087, 1995.
3. P.J. Cornbleet and N. Gochman, "Incorrect Least-Squares Regression Coefficients in Method Comparison
Analysis," Clinical Chemistry 25:3, 432 (1979).
4. Corriveau, Donna: Fritsma, George (ed.): Hemostasis and Thrombosis in the Clinical Laboratory. Ed,
J.B. Lippinncott Company, Philadelphia, 1988, pp 70-71.
i-STAT is a registered trademark of the Abbott Group of Companies in various jurisdictions. Celite is a registered trademark of Celite Corporation,
Santa Barbara, CA, for its diatomaceous earth products. Hemochron is a registered trademark of International Technidyne Corporation, Edison, NJ.
Rev. Date: 01-Jul-13
4
Art: 715878-01M
Kaolin ACT - 7