Abbott i-STAT 1 System Manual page 475

The analyzer should remain on a level surface with the display facing up during testing. If the analyzer is not
level, the ACT result may be affected by more than 10 %. A level surface includes running the handheld in
the downloader/recharger.
Hemodilution may affect test results.
Platelet dysfunction, hereditary or acquired, may affect the results of this test. This includes the
administration of pharmacological compounds known as platelet inhibitors which affect platelet function.
Factor deficiencies, dysprothrombinemias, other coagulopathies, and other pharmacological compounds
may also affect the results of this test.
The i-STAT ACT test is not affected by hematocrit in the range of 20 - 70 %, fibrinogen concentration in the
range from 100 - 500 mg/dL, or sample temperature from 15 - 37ºC.
Specimen Collection and Preparation
The i-STAT ACT test can be performed using venous or arterial samples.
Celite
Venipunctures and Arterial Punctures
• Collection technique resulting in good blood flow must be used.
The sample for testing should be drawn into a plastic collection device (either a plastic
•
syringe or plastic evacuated tube).
The collection device cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
•
• The collection device cannot contain clot activators or serum separators.
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is required, a fresh sample must be obtained.
Note: Some experts recommend drawing and discarding a sample of at least 1 mL prior to drawing
sample for coagulation testing.
Indwelling line
• Fluid drip through the line must be discontinued.
• If blood must be drawn from an indwelling line, possible heparin contamination and specimen dilution
should be considered. The line should be flushed with 5 mL of saline and the first 5 mL of blood or
six dead space volumes should be discarded.
• Withdraw the sample for testing into a fresh plastic syringe.
• The collection syringe cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is needed, draw a fresh sample.
Extracorporeal line
• Flush the extracorporeal blood access line by withdrawing 5 mL of blood into a syringe and discard the
syringe.
• Withdraw the sample for testing into a fresh plastic syringe.
The collection syringe cannot contain anticoagulants such as heparin, EDTA, oxalate, or citrate.
•
• The sample should be immediately dispensed into the sample well of a cartridge.
• If a second measurement is needed, draw a fresh sample.
Rev. Date: 01-Jul-13
5
Art: 714185-01P
Celite ACT - 7