Abbott i-STAT 1 System Manual page 171

Fill Requirements
Mixing
Art: 714372-01M
i-STAT cTnI and CK-MB cartridges require the use of either:
1. heparinized whole blood or plasma samples collected in syringes or
evacuated tubes containing lithium or sodium heparin, or
2. non-heparinized whole blood samples tested within one minute of
drawing from a patient into a plastic syringe or plastic evacuated tube
containing no additives.
The use of whole blood or plasma samples containing other
anticoagulants such as EDTA, oxalate, and citrate will cause
deactivation of the alkaline phosphatase, resulting in decreased cTnI
or CK-MB readings.
i-STAT Total β-hCG cartridges require the use of either:
1. heparinized whole blood or plasma samples collected in plastic
syringes or evacuated tubes containing lithium or sodium heparin
filled to capacity, or
2. non-heparinized whole blood samples tested within one minute of
drawing from a patient into a plastic syringe or plastic evacuated tube
containing no additives.
The use of whole blood or plasma samples containing other
anticoagulants such as EDTA, oxalate, and citrate will cause
deactivation of the alkaline phosphatase, resulting in decreased
β-hCG readings.
i-STAT CHEM8+ cartridges require the use of:
1. whole blood collected in non-heparinized evacuated tubes, or
syringes, as long as sample is tested immediately upon draw,
2. heparinized whole blood collected in balanced heparin syringes or
capillary tubes, or
3. heparinized whole blood collected in evacuated tubes containing
lithium heparin, as long as the tubes are filled to capacity.
Fill blood collection tubes with and without anticoagulant and syringes with
anticoagulant to capacity. Incomplete filling of anticoagulated tubes and
syringes will cause higher heparin-to-blood ratios, which will decrease ionized
calcium results and may affect other results. Under filling blood collection
tubes with and without anticoagulant may also cause decreased PCO
and TCO results.
2
Partial-draw blood collection tubes (evacuated tubes that are adjusted to draw
less than the tube volume, e.g. a 5 mL tube with enough vacuum to draw only
3 mL), with or without anticoagulant, are not recommended for blood gas or
CHEM8+ cartridge analysis because of the potential for decreased PCO
HCO and TCO results. Care must also be taken to eliminate "bubbling" of
3 2
the sample with a pipette when filling a cartridge to avoid the loss of CO
the blood.
Gently mix blood (whether anticoagulated or not) immediately to avoid
clotting. Invert a blood collection tube at least 10 times. Roll a syringe
vigorously between the palms for at least 5 seconds each in two different
directions, then invert the syringe repeatedly for at least 5 seconds, then
discard the first two drops of blood. Note that it may be difficult to properly
mix a sample in a 1.0 cc syringe.
Rev. Date: 27-Jul-16
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