Condenser; Objectiveandceondensercombinations; E Field Diaphragm Nın Ej] Stage Оа - Nikon ECLIPSE E600 Instructions Manual

3. Operation of Each Part
3. Objective and condenser combinations
|
C -
ondenser та Swing-out Achromat Abbe 00 .
Objective aplana condenser condenser condenser magnification
magnification condenser condenser
1x x x x x
2x x x x © (Note 2)
© (Note 3)
4x x O (Note 1) | O (Note 1)
10x to 100x © O O O x
© :Optimum ©: Appropriate — X: Inappropriate
Note 1: The entire view field may not be satisfactory when a UW eyepiece is used.
Note 2: Indoor lighting and similar light sources may be reflected from the surface of the condenser
lens and be visible within the view field. When this happens, either dim the indoor lighting
or prevent strong outside light from entering the stage.
Note 3: Swing out the top lens before usage.
¢ Depending on the type of objective, the whole numerical aperture of the objective may not be
covered. For example, when an objective with an N.A. of 1.4 is used, the aperture size of the
swing-out condenser or the abbe condenser will only be about 65% of the objective's N.A. even
when it is fully opened.
* Refer to the condenser instruction manual for details of universal and phase contrast condensers.
6 Field diaphragm
The field diaphragm restricts illumination to the area on the specimen being viewed. Turning the
field diaphragm ring changes the size of the field diaphragm. For normal observation, the size of the
diaphragm should be slightly larger than the boundary of the view field. If a broader area than
necessary is illuminated, stray light will enter the view field, creating flaring and reducing the con-
trast of the optical image. The correct setting of the field diaphragm is especially important in
photomicrography; generally, good results are obtained by stopping down the field diaphragm to
just slightly larger than the area that will be reproduced on the film, i.e., the size of the photo frame.
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