Focusing And Centering - Nikon ECLIPSE E400 POL Instructions Manual

5 | Condenser
1. Focusing and centering
Image of field diaphragm Image of field diaphragm
Eyepiece view field Eyepiece view field
Focus and center the condenser so that the light passing through the condenser forms the image
the correct position on the specimen (i.e., at the center of the optical path).
Before focusing and centering the condenser, perform steps lll to [B] in chapter "2 Microscopic
Procedure" so that the specimen is in focus with the 10x objective.
1 Close the field diaphragm to its minimum setting.
3 Make rough adjustments with the condenser centering screws so that the image of the field
diaphragm appears at the center of the eyepiece view field.
4 Move the 40x objective into the optical path. Turn the fine focus knob to focus on the
specimen.
6 Adjust the condenser centering screws so that the image of the field diaphragm appears at
the center of the eyepiece view field. This adjustment is easier to make if you adjust the
size of the field diaphragm to be slightly smaller than the eyepiece view field.
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