Focusing And Centering The Condenser; Adjusting The Aperture Diaphragm - Nikon Eclipse LV100POL Instructions Manual

10

Focusing and Centering the Condenser

At the first time usage of the microscope or after attaching the condenser, focus and center the
condenser so that the light through the condenser is focused on the correct position of the specimen
surface (at the center of the optical path).
1 Perform steps in Page 12, "1 Diascopic Bright-
Field Microscopy," and focus on the specimen
using the 10x objective.
2 Turn the field diaphragm control on the
microscope base to stop down the field
diaphragm.
3 Turn the condenser focus knob to form the
field diaphragm image on the specimen
surface.
4 Turn the condenser centering screws (on both
sides) so that the field diaphragm image is
positioned in the center of the view field.
5 Locate the 50x objective into the optical path.
Turn the fine focus knob to focus on the
specimen.
6 Turn the condenser focus knob to form the
field diaphragm image on the specimen
surface.
7 Adjust the field diaphragm control and the
condenser centering screws so that the field
diaphragm image inscribes the view field.
8 To observe the specimen, turn the field
diaphragm control so that the field diaphragm
image circumscribes the view field. (Adjust the
field diaphragm image every time when
objectives are changed.)
11

Adjusting the Aperture Diaphragm

The aperture diaphragm for the diascopic microscopy is adjusted with the condenser aperture
diaphragm ring.
For the aperture diaphragm adjustment for the episcopic microscopy, refer to Page 42, "22
Episcopic microscopy."
30
Field diaphragm image
Eyepiece view field
0
Ach r
0. 8 0.90
0.6 JAPAN
0.4
0.2
F.S.
2
3 6
Condenser
focus knob
EPI /DIA
NC B
ND 8
Field diaphragm
7
control
ON
CLICK
JAPA N
Ach r 0.90 0. 2
0.6 0.4
0.8
OFF
4 7
Condenser
centering
screws