Focusing And Centering The Condenser - Nikon Eclipse Ni-E Instruction Manual

7

Focusing and Centering the Condenser

Adjust the condenser position so that the light passing through the condenser forms an image at the correct position
(center of the optical path) on the surface of the sample.
(1) Follow Steps 1 through 11 in Chapter 2, 3 "Bright/Dark-field
Microscopy Procedure" to focus on the sample using the
10x objective.
(2) Press the right side (
diaphragm button to stop down the field diaphragm to
minimum diameter.
(3) You can see the diaphragm image in the field of view as you
look into the eyepiece. Use the condenser focus knob to
adjust so that the field diaphragm image can be outlined
clearly.
(4) Adjust the condenser centering screws until the field
diaphragm image is at the center of the eyepiece field of
view.
(5) Set the 40x objective into the optical path. Bring the 40x
objective into the optical path. As you see the field
diaphragm image, if the outline of the image is out of focus,
use the condenser focus knob to adjust the focus as much
as possible.
(6) Operate the DIA field diaphragm button to adjust the image
so that the field diaphragm image size is almost the same
as the field of view.
(7) If the center of the field diaphragm image is not centered,
turn the condenser centering screws to move the field
diaphragm image to the center of the field of view. This is
easiest if you adjust the field diaphragm aperture so that it is
slightly smaller than the eyepiece field of view.
Oil type condenser
When using the D-CUO DIC Condenser (Oil), apply
immersion oil between the slide glass and the tip of the
condenser lens before focusing the condenser.
When a slider, to which the DIC module is attached, is
inserted, wipe off the oil.
Correct focusing of the field diaphragm image
If the outline of the field diaphragm image is reddish or bluish, you have turned the condenser focus knob too much.
When the outline is colorless, focusing is correct.
Field diaphragm image view with the 40x objective
The field diaphragm image that has been focused with the 40x objective can not be seen so clearly as the one focused
with the 10x objective.
Chapter 3
Individual Operations
mark side). Of the DIA field
Field diaphragm image
Eyepiece field ring
Minimizing the field diaphragm setting
P A _ _ 2 x _ _ _ _
B I N O _ _ _ _
Focusing the field diaphragm image and moving it
to the center of the field of view
40x
Focusing the field diaphragm image
and opening the field diaphragm wider
100
NC B
ND
ND 11
32
8
OU T
IN
(2)
DIA field diaphragm
button
Z : _ _ _ _ _ 0 . 0 0 0 u m
Z M : 1 . 0 x _ _ F S 1 . 5 m m
Field diaphragm
minimized
(5)
Condenser
focus knob
NC B
ND
ND
32 11
8
OU T
IN
(6)
DIA field diaphragm
button
Field diaphragm image
Eyepiece field ring