Qiagen QIAcuity 911000 User Manual page 280

Mechanical
a) Frame of instrument is distorted (e.g.,
uneven, unstable or not level)
b) Scratches appear on the instrument
Electronic
a) Display does not turn on
b) Error when copying files to USB
c) USB device not detected
d) Login screen not visible when starting
instrument
e) Error displayed when inserting the
USB stick into a Windows PC
f) Starting of instrument takes long
Application
a) Images or analysis data cannot be
viewed
b) Poor or no amplification
c) No clear separation between positive
and negative partitions
d) Images are saturated
e) Sample result is 0 copies/µl or infinite
in absolute quantification
f) Sample results of replicates differ a lot
QIAcuity User Manual 06/2022
Ensure that the instrument is placed on a stable, flat and level
surface as described in Installing the QIAcuity.
Do not touch the display with excessive force or use corrosive
chemicals to clean the display surface.
Contact QIAGEN Technical Services for repair.
Power OFF the QIAcuity, wait for a few minutes, and power it
ON again. Save the file(s) to the USB stick again. Check the USB
stick on a PC to ensure it is functional. If the error persists, contact
QIAGEN Technical Services.
Power OFF the QIAcuity, wait for a few minutes, and power it
ON again. Insert the USB stick into the USB port. Check the USB
stick on a PC to ensure it is functional. If the error persists, contact
QIAGEN Technical Services.
If the touchscreen does not display the login screen, but instead a
software update message is shown, power OFF the QIAcuity and
wait for a few minutes. Ensure that the USB stick is not inserted in
the USB port. Power ON the QIAcuity again. The login screen
should be visible. If the error persists, contact QIAGEN Technical
Services.
After Update of the instrument software the Firmware Update
might be run in the background causing a long starting period. (
up to 60 minutes)
Check the connection to the QIAcuity instrument.
Check if the correct protocols and reagents have been used.
Check if the reaction was set up correctly.
Check the cycling and imaging conditions.
Check if correct restriction enzyme was used when using gDNA
as template material.
Check the starting quality and quantity of the template. We
recommend that you use QIAGEN kits for sample preparation.
Check if the correct protocols and reagents have been used.
Check if the reaction was set up correctly.
Check the cycling and imaging conditions.
Check if correct restriction enzyme was used when using gDNA
as template material.
Check the starting quality and quantity of the template. We
recommend that you use QIAGEN kits for sample preparation.
Re-image the plate with 30% lower exposure duration (see also
section Image quality control)
If your concentration is 0 copies/µl, although the sample is not an
NTC, check the Histogram or 1D Scatterplot for this well. In case
you have nearly only positive partitions in the well, a proper auto-
threshold setting was likely not possible. Please check also if the
image of the well is too dark, and in case re-image the plate with
30% higher exposure time or gain settings.
Check the images for blacked-out areas, that can occur, e.g., due
to bad filling or areas of low amplification (see section Image
corrective measures)
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