Qiagen QIAcuity 911000 User Manual page 225

Concentration diagram tab for genome editing
The Concentration diagram tab shows a diagram that displays the distribution of concentration
values in the wells together with their confidence intervals A concentration diagram has two axes.
The x-axis shows the analyzed wells and samples, and the y-axis represents the concentration
values. The y-axis scale can be modified using the buttons located below each graph. The buttons
are visible when you hold the pointer over a graph. To view the values on a linear scale, click lin.
To view the values on a logarithmic scale, click log10.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to Diagram
options.
Each combination of wild type target and edited target in one sample throughout the selected wells
is represented in the diagram with one bar showing the concentration value together with the
confidence interval. To view detailed information, hold the mouse pointer over its corresponding
bar. A tooltip with detailed information opens.
To view the mean values for replicates on the concentration diagram, click Show mean values for
replicates. If the user clicks into the checkbox to select the mean representation for replicates, the
bars of individual replicates disappear and only one bar is shown at the sample label that represents
the mean edited fraction value of the replicates. When there are no replicates within selected wells
for edited target the bars don't change. The corresponding well IDs of the replicates are shown on
the x-axis. This is also applied in same way by default to the view where targets are in different
reaction mixes. In this case individual replicate results are not available. Mean values are calculated
and shown over all replicates that are included in the well selection only. If there are further
replicates of the same sample that are not included in the well selection, they are not considered in
the calculated mean value and show their individual results values.
QIAcuity User Manual 06/2022 225