Qiagen QIAcuity 911000 User Manual page 216

List tab for mutation detection
The List tab contains a table with an overview of the analyzed wells. The following columns are
available in the table.
Well ID – This column represents the well position in the plate layout.

Sample/NTC/Control – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control.
Reaction mix – This column contains the icon and the name of the Reaction mix..

Target – This column shows all target names with its corresponding target type. Targets which

were selected as wild type or mutant are marked
Concentration (copies/ul) – This column shows the concentration assigned to each target.

CI (95%) – This column shows the value of the confidence interval at a 95% confidence level.

Mutation fraction – This column shows the Mutant fraction value in %

CI (95%) – This column shows the value of the confidence interval for mutant fraction at a 95%

confidence level.
Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured wildtype and mutant targets are part of the same reaction mix):

Replicates are analyzed as separate wells but in addition the mean concentration and mean
mutant fraction values together with the corresponding mean CI are provided on demand.
On the right side above the table there is checkbox which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 4 columns indicating
following mean values:
Mean concentration value

CI (95%) – CI of mean concentration as percentage

Mean mutation fraction as percentage

CI (95%) -– CI of mean mutation fraction as percentage

QIAcuity User Manual 06/2022
accordingly.
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