Qiagen QIAcuity 911000 User Manual page 235

The Concentration diagram is a bar plot which presents two values:
Concentration value as bar

CI value as error bar

The diagram consists of all possible combinations. Each sample on the diagram is represented by
the number of bars according to targets that was selected as a target of interest and reference
targets. Samples on the diagram are sorted by the sample ID. Target bars in the sample show one
concentration bar for target of interest at first position and concentration bars for reference targets
sorted by their well id. The bar color reflects the color assigned to the corresponding targets. The
sample ID is shown center-aligned below the bars.
The Bar size is fixed if:
The reference targets and target of interests are situated on one well there is one well label for

all that belongs to the same well.
The reference targets and target of interests are situated on two wells or more wells there are

one well label per targets that are within this well.
When hovering over a bar in the diagram, a tooltip appears presenting details about the actual
values of concentration and CI and results of tests.
To view the mean values for replicates on the diagram, click Show mean values for replicates.
When this checkbox is checked, the concentration diagram shows bars that represents mean
concentration value for replicates within the well selection. The label below the bar shows well
positions that are included in the replicates value of mean concentration. On hover on the bar,
there is a tooltip with details about wells, sample and target that are part of replicate group and
the results are shown as mean result values with a corresponding mean-label. This is also applied
in same way by default to the view where targets are in different reaction mixes. In this case
individual replicate results are not available. When selected well has no replicates within all
selected wells, individual result values are shown.
Note: If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the "Show mean values for
replicates" check box is marked and can't be changed. In this case there is a warning message
above the diagram title and when the user hover on the checkbox, a tooltip appears to inform the
user that selected analysis source contains replicates of the reference sample and result is calculated
only by using mean results for replicates.
QIAcuity User Manual 06/2022 235