Qiagen QIAcuity 911000 User Manual page 182

Signal map of an image showing areas of bad filling
Crosstalk Correction algorithm
Depending on the optical configuration of your QIAcuity instrument, you can detect up to two or
five fluorescent channels. To compensate the spectral overlap between the fluorescent dyes, a
crosstalk correction algorithm is implemented in the QIAcuity Software Suite. The correction is done
automatically by the software and does not require any user action. The bleed-through signals are
removed from the images and are not taken into account in the result analysis. The crosstalk
correction is correcting an absolute value based on the RFU level of the neighbor channel.
Note: In case insufficient compensation or overcompensation is seen (e.g., as double negative
bands), check the RFU level of positive signals of neighbor channels if saturated or very bright. By
lowering the RFU level of positive signals, the insufficient compensation or overcompensation might
be reduced.
QIAcuity User Manual 06/2022 182