Qiagen QIAcuity 911000 User Manual page 261

therefore has no impact on the result. Refer to the Application guide on www.qiagen.com for
the recommended restriction enzymes.
Important: Do not pipet master mix and sample separately into the Nanoplate as this will lead
to insufficient mixing.
2. Pipet each reaction mix from the pre-plate into a well of the Nanoplate. If possible, use an
electric one-channel pipette. To ensure bubble-free pipetting, we recommend to pipette 39 µl
for Nanoplate 26K 8/24-well, and 11 µl for Nanoplate 8.5K 96/24-well of your prepared
reaction mix to the bottom of the respective input well of the Nanoplate. Ensure not to pipet
into the output well instead of the input well.
Note: To avoid damaging the optical surface and to reduce dust that will interfere with the
imaging and analysis of results, we recommend to place the Nanoplate into a Nanoplate tray
before pipetting the reaction mix into the Nanoplate.
Note: Do not centrifuge the Nanoplate as this will lead to pre-priming and insufficient filling of
the wells.
Note: Do not vortex the Nanoplate as this will lead insufficient filling of the wells.
3. Apply the plate seal that comes with the Nanoplates as follows to ensure good filling of the
wells and to prevent evaporation and contamination:
4. The stiff plate seal consists of a plate seal and 2 protective foils. The 3 layered foil should not
be folded. Remove the bottom white protective foil carefully, center and align the plate seal
(still containing the upper protective foil) with the lower edge of the colored frame of row H.
The foil should not overlap on any side more than 1mm, otherwise the Nanoplate might not
be processed by the instrument. In case the plate seal is incorrectly placed or the seal does
not cover some parts of the Nanoplate, carefully remove this seal and repeat sealing step with
a new one. Correct sealing of the Nanoplate prevents samples from not being fully processed.
Note: It is recommended to cover the plate within 30 minutes after pipetting with the top seal
to prevent subsequent filling issues.
5. After correct placing, the plate seal has to be fixed with the Nanoplate roller in the horizontal
and vertical direction.
6. Afterwards, the upper protective foil is removed on the bottom left corner. We recommend
that one finger holds the rubber foil on the plate corner in place while the upper transparent
foil is pulled off. If the upper foil would be pulled off in another way, the plate seal might get
loose.
7. Use the Nanoplate roller with high force to fix the plate seal on the Nanoplate by rolling at
least 3 times forwards and backwards in horizontal, and also 3 times forwards and
backwards in the vertical direction over the edge of the plate. Roll over the plate seal covering
the Nanoplate frame. The proper fixing of the plate seal is important for a good filling of the
wells.
QIAcuity User Manual 06/2022 261