Qiagen QIAcuity 911000 User Manual page 215

Mutation detection
The Plate Analysis environment of the Software Suite includes the Mutation Detection tab. Mutation
Detection analysis is based on the concentrations (see Absolute quantification). To use Mutation
detection, the definition of targets in the reaction mixes and samples is mandatory. For more
information, see section Setting up an experiment.
The Mutation Detection tab is used to show the results of analyzing plate contents to detect mutations
in the samples. The analysis results are put into list views, heatmaps, point diagrams, and
concentration diagrams.
Note: Saving of mutation detection tests is not available.
Setting up a mutation detection analysis
1. Click the relevant wells in the Select wells pane. For more information, see Selecting wells for
analysis.
2. Select the applicable wild type target from the Wild type target list.
3. Select the applicable mutant target from the Mutant target list.
4. To view the results of the analysis, click Show results.
5. The results are divided into several tabs. To view the contents of the tab, click the tab title.
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