Qiagen QIAcuity 911000 User Manual page 237

Well ID – This column represents the well position in the plate layout.
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Sample/NTC/Control Name – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control. Reference samples are marked with the word Ref.
Reaction mix – This column contains the icon and the name of the Reaction mix.

Target – This column shows all target names with its corresponding target type. Targets which

were selected as target of interest (TOI) or reference target (Ref) are marked accordingly.
Concentration (copies/ul) – This column shows the concentration assigned to each target.
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CI (95%) - This column shows the value of the confidence interval at a 95% confidence level.

Fold change – This column shows the change in the level of gene expression in the sample.

CI (95%) – This column shows the value of the confidence interval for the fold change at a

95% confidence interval.
Fold regulation – This column shows the change in the level of gene expression compared to

the reference sample.
Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured target of interest and reference targets are part of the same reaction

mix):
On the right side above the table there is checkbox which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 5 columns indicating
following mean values:
Mean concentration value

CI (95%) - CI of mean concentration as percentage

Mean fold change

CI (95%) - CI of mean fold change as percentage

Mean fold regulation

Simplex test (configured target of interest and reference targets are part of different reaction

mixes):
QIAcuity User Manual 06/2022 237