Qiagen QIAcuity 911000 User Manual page 180

Improved dust detection for dust causing light infections of neighbor partitions: left, not sufficient dust removal; right, sufficient
dust removal with QIAcuity Software version 2.1. Affected area is removed from analysis and therefore partitions are
invalidated.
Low amplification areas
In case of low amplification areas, the fluorescence signal in the target channels is less pronounced
or not detectable in certain areas of the well. The signal in the reference channel is not affected. If
an equal amplification did not take place in the well, the area of low amplification does not meet
the requirements for Poisson distribution. Therefore, the partitions in those areas are blacked out in
the image and are not included in the analysis.
QIAcuity User Manual 06/2022 180