Qiagen QIAcuity 911000 User Manual page 19

Scheme of filling and partitioning of a well.
Principle of priming and rolling to allow partitioning of the wells.
Thermocycling: The second step and module is a high-accuracy plate thermocycler that performs
the polymerase chain reaction. The cycling profile can be set in the QIAcuity Software Suite or the
instrument software. For more details on the thermal cycler specification, see Appendix A –
Technical Data.
Imaging: The final process step is the image acquisition of all wells. The user can select the detection
channels in the experiment setup. The partitions that have a target molecule inside emit fluorescence light
and are brighter than the ones without target (see image hereafter). For more details and specifications
on the imaging system, see section Appendix A – Technical Data.
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