Qiagen QIAcuity 911000 User Manual page 185

For the analysis, hyperwells are treated as a single well but with a larger number of partitions. This
may be helpful for rare event detection if the sample volume to be analyzed exceeds the volume
that can be loaded into a single well. Results from all wells coupled into a hyperwell will be
aggregated and presented as a result from a single well.
Details about CI calculation and concentration determination in hyperwells are described in the
QIAcuity User Manual Extension: QIAcuity Application Guide available at wwwqiagen.com.
Hyperwells can be selected for Absolute Quantification, Mutation Detection, and Genome Editing,
Copy Number Variation, and Gene Expression analysis.
Using Hyperwells in Copy Number Variation and Gene Expression analysis
1. Select reference sample from the drop-down list.
QIAcuity User Manual 06/2022 185