Qiagen QIAcuity 911000 User Manual page 150

Cycling tab
You can define the temperature profile of your experiment in the Cycling tab. To do this, follow
these steps:
1. In the Temperature field, specify the temperature of the step, then specify the duration of the
temperature step in the Duration field.
2. Click Add Temperature Step.
3. The temperature step is added to your cycling profile.
4. Repeat steps 1–2 for all temperature steps.
Note: Use the up and down arrows to arrange the order of the temperature steps.
5. Check the box corresponding to the temperature steps that you want to use for the repeated
cycling. Then, click Group.
6. In the first column of the grouped temperature steps, add the number of cycles.
Note: To separate the grouped temperature steps, check the box corresponding to the group,
then click Ungroup. To delete a temperature step, check the box corresponding to the step,
then click Delete. The three-dotted icon in each temperature step enables you to edit or delete
the step.
Important: You can enter values for the temperature between 40°C and 99°C.
QIAcuity User Manual 06/2022 150