Qiagen QIAcuity 911000 User Manual page 188

3. Then, select the target of interest. In the example below, the user has chosen SARS-COV-2
with channel Green.
4. The reference selection is available. From the drop-down list are two options: "01 HW2"
(hyperwell with selected wells: A3, B3) or "non-hyperwell, all replicates".
In this case, the user has selected "01 HW2".
5. Next, please select reference target(s).
Here, the user has chosen ERBB2 with channel Yellow.
6. If all fields have been selected, you can click the Show results button.
Multiple imaging steps
If the plate was configured with multiple imaging steps, you can select the one used for analysis.
To select the imaging step, click one of the available boxes indicated by this icon:
channels that were activated in the selected imaging step are shown below the imaging step
buttons, along with the duration and gain.
Important: When switching imaging steps, the application will ask to ungroup existing hyperwells.
All wells linked as hyperwells will be ungrouped as individual wells.
QIAcuity User Manual 06/2022
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