Qiagen QIAcuity 911000 User Manual page 208

Changing the threshold individually per well
1. To change the threshold individually per well, click on the appropriate header of the well in
the 1D Scatterplot view. A window opens and the threshold can be changed by pointing over
the chart, which triggers the appearance of a dotted line.
2. Once the dotted line is in the appropriate spot, click the chart. The line becomes solid, and
the threshold value is updated and shown in the Threshold field.
3. To change the value again using this method, click the red line, and drag it to the appropriate
spot. Alternatively, you can also directly edit the value in the Threshold field Use the auto-
threshold button to set the threshold to the value, which is calculated by the analysis
algorithm.
Note: The Threshold field and the auto Threshold button become only visible by moving the
pointer in the range of the well diagram.
4. Click Recalculate to trigger the re-analysis of data and to close the window. Click Cancel to
close the window without any changes.
Note: To add a single well diagram to the report, click Add to report. For more information on
reports, see Reports.
QIAcuity User Manual 06/2022 208