Qiagen QIAcuity 911000 User Manual page 212

For each partition point the assignment to its group (++, +−, −+, or −−) is derived from the threshold
values. The partition number of each group is displayed on the top right, next to the diagram. The
color of the point that represents the partition fluorescence measurement is assigned according to
the group. A legend is shown on the left side below the 2D Scatterplot view:
On the 2D Scatterplot, only valid partitions are shown.

Dark blue: Partition is assigned for x-axis and y-axis channel as positive (++).

Orange: Partition is assigned to x-axis channel as negative and for y-axis channel as positive

(−+).
Light blue: Partition is assigned to x-axis channel as positive and for y-axis channel as

negative (+−).
Grey: Partition is assigned for x-axis and y-axis channel as negative (−−)

The number of invalid partitions is shown in the legend at the right-hand side.

Invalid partitions on X mean the number of invalid partitions assigned to the x-axis.
Invalid partitions on Y mean the number of invalid partitions assigned to the y-axis.
2D Scatterplot view with 2 Common thresholds.
QIAcuity User Manual 06/2022

212