Qiagen QIAcuity 911000 User Manual page 213

Assigning partitions to a group using free hand (Lasso mode)
The user can select Lasso partitions measurement dots with a free hand mode, so that he can use a
more precise way to assign partitions to the group they should belong to.
The lasso is available in menu above the diagram on hover. When the user clicks lasso icon ( )
he can first select the group (++, +−, −+, or −−) to which partitions should be assigned using the
free hand mode. The points selection is additive. User can add points to the group he selected. If
user assigns one group to a point and afterwards assigns another one, the last one is valid.
After selecting points by lasso, the user can set thresholds only by clicking the auto-threshold icon.
Clicking this icon reactivates the threshold behavior. The user can use either the lasso mode or
threshold setting. A combination of both is not possible. When thresholds are changed and not
confirmed by clicking the Recalculate button, and if some partitions are selected through lasso mode
and recalculated, the threshold values are ignored.
Note: Lasso selection in 2D Scatterplot affects also histogram and 1D Scatterplots views. For details
refer to chapters Histogram tab for absolute quantification and 1D Scatterplot tab for absolute
quantification.
2D Scatterplot – Lasso mode – Group selection before free-hand assignment.
Use the Auto-threshold button for each axis to re-set the threshold to the values, which is calculated
by the analysis algorithm for each well. Click the Recalculate button to trigger the re-analysis of
data.
If you change Common thresholds or the assignment of partitions using the Lasso mode and wants
to leave the 2D Scatterplot view without triggering the Recalculate button a propriate warning
message windows opens. You can continue ignoring your changes or stay in the 2D Scatterplot
view.
To add the 2D scatterplot to the report, click Add to report. For more information on reports, see
section Reports.
QIAcuity User Manual 06/2022 213