Qiagen QIAcuity 911000 User Manual page 174

Note: Errors affecting single wells or empty wells are indicated by a symbol in the affected
well. Try reimaging the plate with different exposure and/or gain settings. If the error persists,
create a support package and contact QIAGEN Technical Services.
Image quality control
The fluorescence signal in the reference channel is measured to determine the number of valid
partitions in a well. Differences in the signal intensities between partitions are normalized and the
fluorescence signals in the target channels are corrected accordingly.
If the fluorescence signal is saturated in too many partitions of a well in a target channel, a warning
message will be shown to the customer. Furthermore, saturated signals are marked with in
the Results overview. Saturated signals lower the signal-to-noise ratio and can lead to improper
analysis results, e.g., the crosstalk correction algorithm might be affected. The recommendation is
to re-image the plate with 30% less exposure time in the respective channel.
QIAcuity User Manual 06/2022 174