Qiagen QIAcuity 911000 User Manual page 214

Concentration diagram tab for absolute quantification
The Concentration Diagram tab shows the diagrams that display the distribution of concentration
values and confidence intervals. One diagram is created for each selected target or channel. A
concentration diagram has two axes. The x-axis shows the analyzed wells, and the y-axis represents
the concentration values for the selected targets or channels of each well. The values on the y-axis
have two available scales – linear and logarithmic.
To view an additional toolbar that enables you to perform actions related to the diagram, such as
downloading the plot, hold the pointer over the diagram. For more details about the toolbar, refer
to section Diagram options.
The y-axis scale can be modified using the buttons located below each graph. The buttons are
visible when you hold the pointer over a graph. To view the values on a linear scale, click lin. To
view the values on a logarithmic scale, click log10.
Each diagram presents two values for each well – the concentration value, displayed as a bar, and
the confidence interval, displayed as an error bar. To view the exact values, point to one of the
bars. After a concentration diagram loads, the first 32 wells are shown in the diagram. To view
other wells, use the slider located below the diagram.
To view the mean values for replicates on the diagram, click Show mean values for replicates. In
this case one bar is shown for a replicate group showing the mean concentration value and the
mean CI value of the replicate group. To view exact values and a list of wells belonging to the
replicate group point to the associated bar.
To add any of the concentration diagrams to the report, click Add to report next to the relevant
diagram. For more information on reports, see section Reports.
QIAcuity User Manual 06/2022 214