Qiagen QIAcuity 911000 User Manual page 222

Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured wildtype and edited targets are part of the same reaction mix):

Replicates are analyzed as separate wells but in addition the mean concentration and mean
edited fraction values together with the corresponding mean CI are provided on demand.
On the right side above the table there is checkbox, which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 4 columns indicating
following mean values:
Mean concentration value

CI (95%) – CI of mean concentration as percentage

Mean edited fraction as percentage

CI (95%) – CI of mean edited fraction as percentage

Simplex test (configured wildtype and edited targets are part of different reaction mixes):

For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:
Mean concentration value

CI (95%) – CI of mean concentration as percentage

Mean edited fraction as percentage

CI (95%) – CI of mean edited fraction as percentage

The replicate results are shown in one row per replicate group. Individual replicate results are not
available. The checkbox that allows the user to show mean values for replicates is checked and
disabled. To export the list view information as a .csv file, click Export to CSV.
QIAcuity User Manual 06/2022 222