Qiagen QIAcuity 911000 User Manual page 217

Simplex test (configured wildtype and mutant targets are part of different reaction mixes):

For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:
Mean concentration value

CI (95%) – CI of mean concentration as percentage

Mean mutation fraction as percentage

CI (95%) – CI of mean mutation fraction as percentage

The replicate results are shown in one row per replicate group. Individual replicate results are
not available. The checkbox that allows the user to show mean values for replicates is checked
and disabled.
To export the list view information as .csv file, click Export to CSV.
Heatmap tab for mutation detection
The Heatmap tab contains a heatmap that shows the mutant fraction as percentage in each of the
wells. If a well is not selected as a source for the analysis, the value is not displayed on the heatmap
and its background color is gray.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to section
Diagram options.
To view detailed information about a well, point the cursor over the well. A tooltip with detailed
information opens.
To view the mean values for replicates in multiplex tests, click Show mean values for replicates. For
simplex tests the mean values view is always shown for replicates and cannot be disabled.
QIAcuity User Manual 06/2022 217